What is the first thing to do when detecting infections?
Posted: 01 May 2018, 15:29
The first thing to do is always to unmask the lurking latent STD’s (sexually transmitted diseases) and Viruses; in all likelihood, this is what makes a mess in your genitourinary system. These must be detected, and the following analyses are needed.
Blood ELISA, PCR and Inoculation (if possible, with determining sensitivity to the extended spectrum of antibiotics), urethral smears, sperm, prostate secretion for the infections below listed (you should have all tests at independent laboratories):
STD
(urethral smears, sperm, prostate secretion for PCS and Inoculation. Blood for ELISA):
- Chlamydia trachomatis
- Chlamydia pneumoniae / Chlamydophila pneumoniae
- Mycoplasma hominis
- Mycoplasma genitalium (Neither ELISA, nor Inoculation are possible)
- Mycoplasma pneumoniae
- Ureaplasma urealyticum
- Ureaplasma parvum (Neither ELISA, nor Inoculation are possible)
- Trichomonas vaginalis (Microscopy and PCR often fail to detect trichomonas. Direct immunofluorescence and Inoculation with preliminary immune-response provocation are more efficient. InPouch™ TV inoculation of medium is advisable http://biomeddiagnostics.com/clinical/c ... -vaginalis, all the other are not so efficient.
- Neisseria gonorrhoeae (Microscopy, PCR, and Inoculation only. ELISA is unavailable)
- Candida and other parasitic fungi (Microscopy, PCR, ELISA and Inoculation)
- Gardnerella vaginalis (Microscopy, PCR, and Inoculation only. ELISA is unavailable)
- Treponema pallidum (PCR and ELISA only. Inoculation is unavailable)
VIRUSES
(urethral smears, sperm, prostate secretion, and saliva for PCS. Blood for ELISA):
- Herpes simplex virus type 1
- Herpes simplex virus type 2
- Varicella-Zoster virus (type 3 herpes)
- Epstein-Barr virus (type 4 herpes)
- Cytomegalovirus (type 5 herpes)
- Herpes simplex virus type 6
- Herpes simplex virus type 7 (PCR of saliva and blood only, ELISA is unavailable)
- Herpes simplex virus type 8
- Human papilloma virus (HPV) type 21 (types: 6, 11, 16, 18, 26, 31, 33, 35, 39, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) (PCR of urethral smear, sperm, secretion of the prostate only.) ELISA is unavailable.
OPPORTUNISTIC AEROBIC AND ANAEROBIC FLORA (hereinafter, the OPF)
(urethral smear, sperm, secretion of the prostate for Inoculation).
- Aerobic flora: incl. Enterococcus, Streptococcus, Staphylococcus
- Anaerobic flora: incl. Bacteroides spp., Fusobacterium spp., Veillonella spp., Prevotella (P. bivia, P. buccae, P. disiens); anaerobic Gram-positive rods: Clostridium spp., Eubacterium spp., Peptococcus spp., Peptostreptococcus spp. and etc.
Opportunistic pathogenic flora and anaerobic flora are not included in the list of STDs.
Today, the most efficient technique, which can reveal even long-standing STDs is, probably, the DIF (Direct immunofluorescence) only.
The DIF has been undeservedly discredited for today, and removed from the laboratory diagnosis almost everywhere. Actually, DIF is a very effective technique for identifying STDs, it is deliberately prohibited everywhere, because it is very sensitive and can detect STD almost always. To cure people is unprofitable for the today’s medicine, so effective methods of identifying and treating STDs are discredited and prohibited, including DIF, and replaced by rubbish such as PCR. Earlier, in the Soviet era, STD infection used to be identified with DIF only.
At the forum, there are examples of identifying long-standing STDs by the method of direct immunofluorescence, while no other method could detect them, and in the first place, PCR, which is the fullest bullshit. https://hron-prostatit.ru/forum/viewtop ... 5&start=20 (RU)
Possibly, you might find the DIF in municipal hospitals, research institutes, family medicine centers for conception, in women's health centers (men have not been given such an honor, so it's unlikely to find DIF in men's health centers).
As the forum users see it, PCR very often fails to detect anything in most cases.
From experience of the forum member, blood ELISA does not always detect anything either, because STDs shoot through the immune system and, as a result, they will shoot through the antibody response to SRDs, which antibodies ELISA must detect; and if there are no antibodies, ELISA will not detect them, so, yielding negative result. Or, antibodies might be detected, but low-titered, which, according to doctors, is also a negative result.
Inoculations work poor as well, because in the chronic form, STDs almost never proliferate, which means that the culture will not grow, and negative result will be obtained.
Seek where direct immunofluorescence test can be conducted to detect the following:
- Chlamydia trachomatis
- Mycoplasma hominis
- Mycoplasma genitalium
- Ureaplasma urealyticum
- Ureaplasma parvum
- Trichomonas vaginalis
- Neisseria gonorrhoeae
- Gardnerella vaginalis
- Treponema pallidum
- Herpes simplex virus type 1
- Herpes simplex virus type 2
- Varicella-Zoster virus (type 3 herpes)
- Epstein-Barr virus (type 4 herpes)
- Cytomegalovirus (type 5 herpes)
- Herpes simplex virus type 6(DIF is not applied, have PCR test only)
- Herpes simplex virus type 7(DIF is not applied, have PCR test only)
- Herpes simplex virus type 8(DIF is not applied, have PCR test only)
- Human papilloma virus (HPV) (DIF is not applied, have PCR test only)
1. Men should test urethral smears, prostate secretion, and sperm by direct immunofluorescence. Women should have cervical canal, vaginal, and urethral smear tested.
All possible infection sites require testing, that is, all the listed biomaterials should be submitted for all STDs.
2. Also, men had better submit urethral smears, prostate secretion and sperm, while women - cervical canal, vaginal, and urethral smears for Microscopy and Inoculation to detect the following STDs:
- Candida and other parasitic fungi
- Trichomonas vaginalis
- Neisseria gonorrhoeae
- Gardnerella vaginalis
3. I do not know, whether DIF is used to detect papillomavirus – I have found no information. So, use PCR only so far. It is smears only to be tested. This means that men should submit urethral smears, while women – smears from the cervical canal, vagina, and urethral smears. It is up to you, whether to use PCR for the detection of papillomaviruses, because you should have been aware now, what PCR diagnostics is like. There is no other method to identify the same.
4. If you fail to use DIF to detect some or other infection – well, PCR is possible, however, keep in mind that PCR diagnostics leaves much to be desired.
I do not recommend to complete tests under paragraphs 1 and 2, as well as 1 and 4 at the same laboratory, because if, for example, DIF has yielded a positive result, but microscopy or culture, or PCR has yielded a negative one, the facility you have resorted to may “garble” the results, and interpret DIF as negative as well, so as not to be discredited. To that point, have DIF independently.
First, have DIF. Then, if the DIF test shows nothing, inoculation and microscopy is also possible. Anyway, the latter is required to detect fungi.
If any disease is detected by any of the above listed techniques, you have the disease, ten out of ten. If a disease is detected in one of the partners (let us call it “partner A”), but it is not detected in the other partner (“partner B”), be sure, the partner B, without fail, is infected by the same disease, even if the latter complains of nothing, because the infection may be clinically silent. That is, in any case, both partners will have to receive treatment, otherwise, they will infect each other time after time.
Preliminary alcohol challenge may increase the likelihood of detection of a sexually transmitted disease, you also may try beer and smoked food, but not naturally dried fish, because it might cause opistarchosis. By the way, alcoholic challenge is not so great.
Pyrogenal provocation would be more efficient, but this trick is rather dangerous, and you may harm yourself if you do not have necessary experience or you are not certain of a safe dose. Be VERY CAREFUL.
--------------
But!!!
Chlamydia, mycoplasma, ureaplasma, (gonococcus seems to be too) are intracellular infections, and in the chronic form, it is useless to take scrapings from the anterior part of the urethra, since intracells will gradually pass from the anterior urethra to the prostatic section of the urethra, into the prostate, seminal vesicles, epididymis or testicles, the bladder, i.e. upwards. This means that it is useless to take scrapings from the anterior urethra for STD, because the collected material will merely not contain the same, that is, in such a case, any methods of detecting infections and even the methods of DIF and Cytology are useless, the result will be negative.
Further, the following points should be taken into account.
Also, it is useless to take the prostatic fluid, secretion of seminal vesicles and semen (hereinafter referred to as the fluid) for intracells in the chronic form, because if they sit inside the cells and do not proliferate – and they do not proliferate in the chronic form any longer, then they do not go outside in the fluid, so neither prostate secretion, nor seminal vesicles or semen will contain them, i.e. it is useless to submit the materials for testing, despite the fact that the intracells are in the tissues of the prostate, and the seminal vesicles, and the epididymis, and even in the testicles, they do not go beyond the cells of their own tissues.
Therefore, to analyze fluids even by DIF or Cytology is useless, because the collected liquid materials will not contain the infection, so, any method of testing is sure to give negative result.
The only possible method is the scraping from the prostatic part of the urethra with a urological catheter, and preferably alongside of the urethra, or scraping through the urethroscope inserted into the prostatic region.
Blood ELISA, PCR and Inoculation (if possible, with determining sensitivity to the extended spectrum of antibiotics), urethral smears, sperm, prostate secretion for the infections below listed (you should have all tests at independent laboratories):
STD
(urethral smears, sperm, prostate secretion for PCS and Inoculation. Blood for ELISA):
- Chlamydia trachomatis
- Chlamydia pneumoniae / Chlamydophila pneumoniae
- Mycoplasma hominis
- Mycoplasma genitalium (Neither ELISA, nor Inoculation are possible)
- Mycoplasma pneumoniae
- Ureaplasma urealyticum
- Ureaplasma parvum (Neither ELISA, nor Inoculation are possible)
- Trichomonas vaginalis (Microscopy and PCR often fail to detect trichomonas. Direct immunofluorescence and Inoculation with preliminary immune-response provocation are more efficient. InPouch™ TV inoculation of medium is advisable http://biomeddiagnostics.com/clinical/c ... -vaginalis, all the other are not so efficient.
- Neisseria gonorrhoeae (Microscopy, PCR, and Inoculation only. ELISA is unavailable)
- Candida and other parasitic fungi (Microscopy, PCR, ELISA and Inoculation)
- Gardnerella vaginalis (Microscopy, PCR, and Inoculation only. ELISA is unavailable)
- Treponema pallidum (PCR and ELISA only. Inoculation is unavailable)
VIRUSES
(urethral smears, sperm, prostate secretion, and saliva for PCS. Blood for ELISA):
- Herpes simplex virus type 1
- Herpes simplex virus type 2
- Varicella-Zoster virus (type 3 herpes)
- Epstein-Barr virus (type 4 herpes)
- Cytomegalovirus (type 5 herpes)
- Herpes simplex virus type 6
- Herpes simplex virus type 7 (PCR of saliva and blood only, ELISA is unavailable)
- Herpes simplex virus type 8
- Human papilloma virus (HPV) type 21 (types: 6, 11, 16, 18, 26, 31, 33, 35, 39, 44, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82) (PCR of urethral smear, sperm, secretion of the prostate only.) ELISA is unavailable.
OPPORTUNISTIC AEROBIC AND ANAEROBIC FLORA (hereinafter, the OPF)
(urethral smear, sperm, secretion of the prostate for Inoculation).
- Aerobic flora: incl. Enterococcus, Streptococcus, Staphylococcus
- Anaerobic flora: incl. Bacteroides spp., Fusobacterium spp., Veillonella spp., Prevotella (P. bivia, P. buccae, P. disiens); anaerobic Gram-positive rods: Clostridium spp., Eubacterium spp., Peptococcus spp., Peptostreptococcus spp. and etc.
Opportunistic pathogenic flora and anaerobic flora are not included in the list of STDs.
Today, the most efficient technique, which can reveal even long-standing STDs is, probably, the DIF (Direct immunofluorescence) only.
The DIF has been undeservedly discredited for today, and removed from the laboratory diagnosis almost everywhere. Actually, DIF is a very effective technique for identifying STDs, it is deliberately prohibited everywhere, because it is very sensitive and can detect STD almost always. To cure people is unprofitable for the today’s medicine, so effective methods of identifying and treating STDs are discredited and prohibited, including DIF, and replaced by rubbish such as PCR. Earlier, in the Soviet era, STD infection used to be identified with DIF only.
At the forum, there are examples of identifying long-standing STDs by the method of direct immunofluorescence, while no other method could detect them, and in the first place, PCR, which is the fullest bullshit. https://hron-prostatit.ru/forum/viewtop ... 5&start=20 (RU)
Possibly, you might find the DIF in municipal hospitals, research institutes, family medicine centers for conception, in women's health centers (men have not been given such an honor, so it's unlikely to find DIF in men's health centers).
As the forum users see it, PCR very often fails to detect anything in most cases.
From experience of the forum member, blood ELISA does not always detect anything either, because STDs shoot through the immune system and, as a result, they will shoot through the antibody response to SRDs, which antibodies ELISA must detect; and if there are no antibodies, ELISA will not detect them, so, yielding negative result. Or, antibodies might be detected, but low-titered, which, according to doctors, is also a negative result.
Inoculations work poor as well, because in the chronic form, STDs almost never proliferate, which means that the culture will not grow, and negative result will be obtained.
Seek where direct immunofluorescence test can be conducted to detect the following:
- Chlamydia trachomatis
- Mycoplasma hominis
- Mycoplasma genitalium
- Ureaplasma urealyticum
- Ureaplasma parvum
- Trichomonas vaginalis
- Neisseria gonorrhoeae
- Gardnerella vaginalis
- Treponema pallidum
- Herpes simplex virus type 1
- Herpes simplex virus type 2
- Varicella-Zoster virus (type 3 herpes)
- Epstein-Barr virus (type 4 herpes)
- Cytomegalovirus (type 5 herpes)
- Herpes simplex virus type 6(DIF is not applied, have PCR test only)
- Herpes simplex virus type 7(DIF is not applied, have PCR test only)
- Herpes simplex virus type 8(DIF is not applied, have PCR test only)
- Human papilloma virus (HPV) (DIF is not applied, have PCR test only)
1. Men should test urethral smears, prostate secretion, and sperm by direct immunofluorescence. Women should have cervical canal, vaginal, and urethral smear tested.
All possible infection sites require testing, that is, all the listed biomaterials should be submitted for all STDs.
2. Also, men had better submit urethral smears, prostate secretion and sperm, while women - cervical canal, vaginal, and urethral smears for Microscopy and Inoculation to detect the following STDs:
- Candida and other parasitic fungi
- Trichomonas vaginalis
- Neisseria gonorrhoeae
- Gardnerella vaginalis
3. I do not know, whether DIF is used to detect papillomavirus – I have found no information. So, use PCR only so far. It is smears only to be tested. This means that men should submit urethral smears, while women – smears from the cervical canal, vagina, and urethral smears. It is up to you, whether to use PCR for the detection of papillomaviruses, because you should have been aware now, what PCR diagnostics is like. There is no other method to identify the same.
4. If you fail to use DIF to detect some or other infection – well, PCR is possible, however, keep in mind that PCR diagnostics leaves much to be desired.
I do not recommend to complete tests under paragraphs 1 and 2, as well as 1 and 4 at the same laboratory, because if, for example, DIF has yielded a positive result, but microscopy or culture, or PCR has yielded a negative one, the facility you have resorted to may “garble” the results, and interpret DIF as negative as well, so as not to be discredited. To that point, have DIF independently.
First, have DIF. Then, if the DIF test shows nothing, inoculation and microscopy is also possible. Anyway, the latter is required to detect fungi.
If any disease is detected by any of the above listed techniques, you have the disease, ten out of ten. If a disease is detected in one of the partners (let us call it “partner A”), but it is not detected in the other partner (“partner B”), be sure, the partner B, without fail, is infected by the same disease, even if the latter complains of nothing, because the infection may be clinically silent. That is, in any case, both partners will have to receive treatment, otherwise, they will infect each other time after time.
Preliminary alcohol challenge may increase the likelihood of detection of a sexually transmitted disease, you also may try beer and smoked food, but not naturally dried fish, because it might cause opistarchosis. By the way, alcoholic challenge is not so great.
Pyrogenal provocation would be more efficient, but this trick is rather dangerous, and you may harm yourself if you do not have necessary experience or you are not certain of a safe dose. Be VERY CAREFUL.
--------------
But!!!
Chlamydia, mycoplasma, ureaplasma, (gonococcus seems to be too) are intracellular infections, and in the chronic form, it is useless to take scrapings from the anterior part of the urethra, since intracells will gradually pass from the anterior urethra to the prostatic section of the urethra, into the prostate, seminal vesicles, epididymis or testicles, the bladder, i.e. upwards. This means that it is useless to take scrapings from the anterior urethra for STD, because the collected material will merely not contain the same, that is, in such a case, any methods of detecting infections and even the methods of DIF and Cytology are useless, the result will be negative.
Further, the following points should be taken into account.
Also, it is useless to take the prostatic fluid, secretion of seminal vesicles and semen (hereinafter referred to as the fluid) for intracells in the chronic form, because if they sit inside the cells and do not proliferate – and they do not proliferate in the chronic form any longer, then they do not go outside in the fluid, so neither prostate secretion, nor seminal vesicles or semen will contain them, i.e. it is useless to submit the materials for testing, despite the fact that the intracells are in the tissues of the prostate, and the seminal vesicles, and the epididymis, and even in the testicles, they do not go beyond the cells of their own tissues.
Therefore, to analyze fluids even by DIF or Cytology is useless, because the collected liquid materials will not contain the infection, so, any method of testing is sure to give negative result.
The only possible method is the scraping from the prostatic part of the urethra with a urological catheter, and preferably alongside of the urethra, or scraping through the urethroscope inserted into the prostatic region.